This document specifies a method for the determination of the aflatoxins B₁, B₂, G₁ and G₂ in vegetable fats and oils, including peanut oil, sesame oil, olive oil, corn oil, sunflower oil, rapeseed oil and coconut oil, using immunoaffinity column clean-up and high-performance liquid chromatography with post-column derivatization.

The limits of quantification for the aflatoxins B₁, B₂, G₁ and G₂, and for the sum of aflatoxins B₁, B₂, G₁ and G₂, are 1 μg/kg, 0,25 μg/kg, 0,5 μg/kg, 0,25 μg/kg and 1 μg/kg, respectively.

The validation was carried out over the following concentration ranges:

—    

aflatoxin B₁ = 1 μg/kg to 20 μg/kg;

—    

total aflatoxins = 2 μg/kg to 52 μg/kg.